Blar i forfatter "Schuttpelz, Mark"

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    • Chip-based wide field-of-view nanoscopy 

      Ahluwalia, Balpreet Singh; Helle, Øystein Ivar; Diekmann, Robin; Øie, Cristina Ionica; McCourt, Peter A. G.; Schuttpelz, Mark (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-04-24)
      Present optical nanoscopy techniques use a complex microscope for imaging and a simple glass slide to hold the sample. Here, we demonstrate the inverse: the use of a complex, but mass-producible optical chip, which hosts the sample and provides a waveguide for the illumination source, and a standard low-cost microscope to acquire super-resolved images via two different approaches. Waveguides composed ...

    • Cost-efficient nanoscopy reveals nanoscale architecture of liver cells and platelets 

      Mao, Hong; Diekmann, Robin; Liang, Hai; Cogger, Victoria Carroll; Le Couteur, David George; Lockwood, Glen P; Hunt, Nick; Schuttpelz, Mark; Huser, Thomas Rolf; Chen, Vivien; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-09)
      Single-molecule localization microscopy (SMLM) provides a powerful toolkit to specifically resolve intracellular structures on the nanometer scale, even approaching resolution classically reserved for electron microscopy (EM). Although instruments for SMLM are technically simple to implement, researchers tend to stick to commercial microscopes for SMLM implementations. Here we report the construction ...

    • Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies 

      Kong, Cihang; Bobe, Stefanie; Pilger, Christian; Lachetta, Mario; Øie, Cristina Ionica; Kirschnick, Nils; Mönkemöller, Viola; Hübner, Wolfgang; Förster, Christine; Schuttpelz, Mark; Kiefer, Friedemann; Huser, Thomas; Schulte am Esch, Jan (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-17)
      The liver as the largest organ in the human body is composed of a complex macroscopic and microscopic architecture that supports its indispensable function to maintain physiological homeostasis. Optical imaging of the human liver is particularly challenging because of the need to cover length scales across 7 orders of magnitude (from the centimeter scale to the nanometer scale) in order to fully ...

    • Nanoscopy of bacterial cells immobilized by holographic optical tweezers 

      Diekmann, Robin; Wolfson, Deanna; Spahn, Christoph; Heilemann, Mike; Schuttpelz, Mark; Huser, Thomas (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-12-13)
      Imaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizing ...